Vortex-assisted Solid-Liquid Extraction for Rapid Screening of Oil Content in Jatropha Seed: an Alternative to the Modified Soxhlet Method

ABSTRACT In this study, an optimized vortex-assisted method for rapid screening of oil content in Jatropha curcas L. (Jatropha) seeds is described. A 24-1IV fractional factorial design was employed to study the influence vortex stirring rate, sample:solvent ratio, extraction time, and number cycles efficiency. Gas chromatography-mass spectrometry (GC-MS) used identification fatty acids. The most important factor, optimum conditions were two extractions with hexane at a ratio 1:5 mg:μL, under vortex-stirring 2500 rpm 1 min. Linoleic acid, oleic acid palmitic major acids oil. showed inherent advantages simplicity, accuracy, short time analysis, low consumption both organic solvent sample, may serve as cost-effective alternative modified Soxhlet estimation composition breeding programmes. Keywords: Vortex-assisted, oil, design, GC-MS.